FORENSIC BIOLOGY 101
DNA Fingerprinting
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DNA fingerprinting has established itself as an efficient and highly accurate means of determining identities and relationships. It has practically revolutionized the field of forensics, especially concerning rape cases. DNA profiling, as the process is more appropriately called, involves the visualization of special segments of the human genome, which are unique to each individual. These special segments, called Standard Tandem Repeats (STR), can be cut out and separated from the rest of the DNA by two processes: mapping Restriction Fragment Length Polymorphisms (RFLP) and Polymerase Chain Reaction (PCR). After being separated from the other DNA, we can visualize these STR segments and separate them by size using gel electrophoresis. STR sequences can also be directly sequenced using DNA sequencing machines, but this method has yet to move into mainstream usage and is used primarily within the research community. (http://www.usc.edu/dept/engineering/illumin/vol3issue3/dna/index1.html) | ||||